医学公开数据库查询与登记

references/brenda.md

Important: BRENDA uses SOAP, not REST. Requires Python with zeep library.

SOAP Endpoint

https://www.brenda-enzymes.org/soap/brenda_zeep.wsdl

Auth

Free registration required at https://www.brenda-enzymes.org/register.php Credentials (email + SHA-256 hashed password) passed with every call.

Key SOAP Methods

All methods take email, password (SHA-256), and ecNumber as base parameters.

Method	Description
getKmValue	Michaelis constant (Km)
getTurnoverNumber	Turnover number (kcat)
getKcatKmValue	Catalytic efficiency (kcat/Km)
getKiValue	Inhibition constant (Ki)
getIc50Value	IC50 values
getSpecificActivity	Specific activity
getPhOptimum	pH optimum
getTemperatureOptimum	Temperature optimum
getSubstrate	Substrates
getProduct	Products
getInhibitors	Inhibitors
getCofactor	Cofactors
getOrganism	Source organisms
getReaction	Reaction equations
getSequence	Protein sequences
getDisease	Associated diseases

Parameter Syntax

fieldName*value format. Empty value = return all.

ecNumber*1.1.1.1           # Required: EC number
organism*Homo sapiens      # Optional: filter by organism
substrate*ethanol          # Optional: filter by substrate
kmValue*                   # Return field (empty = all)

Python Example

import hashlib
from zeep import Client

client = Client("https://www.brenda-enzymes.org/soap/brenda_zeep.wsdl")
email = "your@email.com"
password = hashlib.sha256("your_password".encode()).hexdigest()

# Get Km values for alcohol dehydrogenase
result = client.service.getKmValue(
    email, password,
    "ecNumber*1.1.1.1", "organism*Homo sapiens",
    "kmValue*", "kmValueMaximum*", "substrate*", "commentary*",
    "ligandStructureId*", "literature*"
)

Response Format

The brenda_zeep.wsdl binding returns typed records, including kmValue, kmValueMaximum, organism, and substrate for getKmValue. Serialize with zeep.helpers.serialize_object when needed. The delimiter-separated string format belongs to the older brenda.wsdl interface; do not apply that parser to Zeep record objects. Each method has its own ordered arguments: inspect its WSDL signature instead of reusing the Km parameter tuple for every method.

Rate Limits

BRENDA requests at most one request per second. The online service is CC BY 4.0; registration is required. The authenticated example is illustrative and was not executed during this documentation review.

Reviewed 2026-09-30: official SOAP help and the linked Zeep WSDL.

Note for this skill

Since BRENDA uses SOAP (not REST), making calls requires writing and executing a Python script with zeep. Use Bash to run the script rather than WebFetch.